The potential for complete automated scoring of the cytokinesis block micronucleus cytome assay using imaging flow cytometry
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چکیده
منابع مشابه
Testing chemical agents with the cytokinesis-block micronucleus cytome assay.
In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimeti...
متن کاملOptimized automated data analysis for the cytokinesis‐block micronucleus assay using imaging flow cytometry for high throughput radiation biodosimetry
The cytokinesis-block micronucleus (CBMN) assay is a well-established technique that can be employed in triage radiation biodosimetry to estimate whole body doses of radiation to potentially exposed individuals through quantitation of the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay has been partially automated using traditional microscope-based methods and mo...
متن کاملThe imaging flow cytometry cytokinesis‐block micronucleus assay for radiation biodosimetry
The cytokinesis-block micronucleus (CBMN) assay is an accepted method for biodosimetry where the dose of radiation to potentially exposed individuals is estimated from the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay is traditionally performed using a microscope-based scoring procedure which can be labour intensive, time consuming and subject to variability of...
متن کاملAutomation of the cytokinesis-block micronucleus cytome assay by laser scanning cytometry and its potential application in radiation biodosimetry.
Here we describe the adaptation of laser scanning cytometry (LSC) to measure micronuclei (MN) automatically in lymphocytes. MN frequencies were determined in irradiated human lymphocytes using the cytokinesis-block technique, and the results from LSC were compared with visual scoring results obtained from slides of cells stained using Fast Green and 4',6-diamidino-2-phenylindole (DAPI). This fl...
متن کاملHUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures.
Criteria for scoring micronuclei and nucleoplasmic bridges in binucleated cells in the cytokinesis-block micronucleus assay for isolated human lymphocyte cultures are described in detail. Morphological characteristics of mononucleated cells, binucleated cells, and multinucleated cells as well as necrotic and apoptotic cells and nuclear buds are also described. These criteria are illustrated by ...
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ژورنال
عنوان ژورنال: Mutation Research/Genetic Toxicology and Environmental Mutagenesis
سال: 2018
ISSN: 1383-5718
DOI: 10.1016/j.mrgentox.2018.05.003